Erythropoietin (EPO) is the major glycoprotein hormone regulator of mammalian erythropoiesis, and is produced by kidney and liver in an oxygen-dependent manner. The biological effects of EPO are mediated by the specific erythropoietin receptor (EPOR/EPO Receptor) on bone marrow erythroblasts, which transmits signals important for both proliferation and differentiation along the erythroid lineage. EPOR protein is a type â… single-transmembrane cytokine receptor, and belongs to the homodimerizing subclass which functions as ligand-induced or ligand-stabilized homodimers. EPOR signaling prevents neuronal death and ischemic injury. Recent studies have shown that EPO and EPOR protein may be involved in carcinogenesis, angiogenesis, and invasion.
Product Name:
Human EPO Receptor/EPOR Recombinant Protein (RPES1218)
Product Code:
RPES1218
Size:
100 µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
EPO-R
Accession:
NP_000112.1
Sequence:
Met-Pro 250
Fusion tag:
C-Fc
Activity:
1. Measured by its binding ability in a functional ELISA. Immobilized CD131 at 10 µg/ml (100 ?l/well) can bind biotinylated recombinant human EPOR with a linear range of 0.16-4 µg/ml.2. Measured by its ability to inhibit EpoÂdependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 1-4 ng/ml in the presence of 0.1 U/mL Recombinant Human EPO.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain (Met-Pro 250) of human erythropoietin receptor (NP_000112.1) precursor was expressed with the C-terminal fused Fc region of human IgG1.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
51.0 kDa
AP Mol Mass:
55-60 kDa
Formulation:
Lyophilized from sterile PBS, 8% sucrose, 0.5% Tween-20, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
