Description
Recombinant Human ErbB-2/HER2/CD340 Protein
The Recombinant Human ErbB-2/HER2/CD340 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating ErbB-2/HER2/CD340 function and its implications in health and disease.
This product (SKU: RPCB0331) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 70.19 kDa with an observed molecular weight of 100-120 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE;≥ 90 % as determined by HPLC.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Human ErbB-2/HER2/CD340 Protein |
| SKU: | RPCB0331 |
| Size: | 10 μg , 20 μg , 50 μg , 100 μg |
| Reactivity: | Human |
| Synonyms: | ERBB2, CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1, MLN19, erb-b2 receptor tyrosine kinase 2, ErbB2, Phospho-ErbB2 (Y1139), CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1, erb-b2 receptor tyrosine kinase 2, HER2/ErbB2, MLN19 |
| Tag: | C-His |
| Expression Host: | HEK293 cells |
| Calculated MW: | 70.19 kDa |
| Observed MW: | 100-120 kDa |
| Gene ID: | 2064 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human ErbB-2/HER2/CD340 Protein (RPCB0331), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 0.1 EU/μg of the protein by LAL method. |
| Purity: | ≥ 95 % as determined by SDS-PAGE;≥ 90 % as determined by HPLC. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| Bio-Activity: | Measured by its binding ability in a functional ELISA. Immobilized recombinant human ERBB2 at 10 ng/mL (100 μL/well) can bind Trastuzumab with a linear range of 4-20 ng/mL. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
Epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. ErbB2 has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.
 | Recombinant Human ErbB-2/HER2/CD340 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
 | The purity of Human HER2/ErbB2 Protein (RPCB0331) was greater than 90% as determined by SEC-HPLC. |
