Recombinant Human Fc-gamma RIII alpha/FCGR3A/CD16a Protein
The Recombinant Human Fc-gamma RIII alpha/FCGR3A/CD16a Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Fc-gamma RIII alpha/FCGR3A/CD16a function and its implications in health and disease.
This product (SKU: RPCB0224) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 22.68 kDa with an observed molecular weight of 45-52 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Human Fc-gamma RIII alpha/FCGR3A/CD16a Protein
SKU:
RPCB0224
Size:
10 μg , 20 μg , 50 μg , 100 μg
Reactivity:
Human
Synonyms:
CD16, CD16A, FCG3, FCGR3, FCGRIII, FCR-10, FCRIII, FCRIIIA, IGFR3, IMD20, FCGR3A, CD16, CD16A, FCG3, FCGR3, FCGRIII, FCR-10, FCRIII, FCRIIIA, IGFR3, IMD20, Fc fragment of IgG receptor IIIa
High quality, high purity and low endotoxin recombinant Recombinant Human Fc-gamma RIII alpha/FCGR3A/CD16a Protein (RPCB0224), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Bio-Activity:
Measured by its binding ability in a functional ELISA. Immobilized Human FCGR3A at 1 μg/mL (100 μL/well) can bind FCGR3A Mouse mAb with a linear range of 0.13-6.1 ng/mL.
Measured by its binding ability in a functional ELISA.Immobilized Human FCGR3A at 1 μg/mL (100 μL/well) can bind FCGR3A Mouse mAb with a linear range of 0.06-7.1 ng/mL.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
This protein is a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. The protein is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia.
Recombinant Human Fc-gamma RIII alpha/FCGR3A/CD16a Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized Recombinant Human CD16A at 5 µg/mL (100 μL/well) can bind Recombinant IgG1 with a linear range of 10-40 µg/mL.
Immobilized Human FCGR3A at 1 μg/mL (100 μL/well) can bind FCGR3A Mouse mAb with a linear range of 0.06-7.1 ng/mL.
