Recombinant Human Fc-gamma RIII beta/FCGR3B/CD16b (S36R, N82D, I106V) Protein
The Recombinant Human Fc-gamma RIII beta/FCGR3B/CD16b (S36R, N82D, I106V) Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Fc-gamma RIII beta/FCGR3B/CD16b (S36R, N82D, I106V) function and its implications in health and disease.
This product (SKU: RPCB0488) is produced using Yeast and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 22.52 kDa with an observed molecular weight of 40-50 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Human Fc-gamma RIII beta/FCGR3B/CD16b (S36R, N82D, I106V) Protein
High quality, high purity and low endotoxin recombinant Recombinant Human Fc-gamma RIII beta/FCGR3B/CD16b (S36R, N82D, I106V) Protein (RPCB0488), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.
Formulation:
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Human Fc gamma RIIIB/CD16b Protein is a receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.
Recombinant Human Fc-gamma RIII beta/FCGR3B/CD16b (S36R, N82D, I106V) Protein was determined by Tris-Bis PAGE under reducing conditions.
The purity of Human Fc gamma RIIIB (NA1) is greater than 95% as determined by SEC-HPLC.
