The Recombinant Human FGF-10 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating FGF-10 function and its implications in health and disease.
This product (SKU: RPCB0828) is produced using E. coli and features a No tag tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 19.32 kDa with an observed molecular weight of 20-25 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human FGF-10 Protein (RPCB0828), tested reactivity in E. coli and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS pH7.4.
Bio-Activity:
Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. The ED 50 for this effect is 2.08-8.32 ng/mL, corresponding to a specific activity of 1.20×10 5 ~4.81×10 5 units/mg.
Human stomach organoids were cultured with EGF, FGF10(RPCB0828), NOG(RPCB0864), RSPO1(RPCB0176), WNT-3a(Cat. RP01618SLQ).
Human liver organoids were cultured with EGF, HGF(RPCB1637), FGF2(RPCB0853), FGF10(RPCB0828), NOG(RPCB0864), RSPO1(RPCB0176), WNT-3a(Cat. RP01618SLQ).
Human kidney organoids were cultured with EGF, FGF2(RPCB0853), FGF7(RPCB1793), FGF9(RPCB1059), FGF10(RPCB0828), IGF-(RPCB0759), NOG(RPCB0864), RSPO1(RPCB0176), WNT-3a(Cat. RP01618SLQ). And further, DKK-1(RPCB0891) was used to induce the establishment of cell polarity.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
This protein is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of lim bud formation. This gene is also implicated to be a primary factor in the process of wound healing.
Recombinant Human FGF-10 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Recombinant Human FGF-10 Protein promotes proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. The ED50 for this effect is 2.08-8.32 ng/mL, corresponding to a specific activity of 1.20×105~4.81×105 units/mg.
Human liver organoids were cultured with EGF, HGF(RPCB1637), FGF2(RPCB0853), FGF10(RPCB0828), NOG(RPCB0864), RSPO1(RPCB0176), WNT-3a(Cat. RP01618SLQ).
Human kidney organoids were cultured with EGF, FGF2(RPCB0853), FGF7(RPCB1793), FGF9(RPCB1059), FGF10(RPCB0828), IGF-(RPCB0759), NOG(RPCB0864), RSPO1(RPCB0176), WNT-3a(Cat. RP01618SLQ). And further, DKK-1(RPCB0891) was used to induce the establishment of cell polarity.
