Description
Recombinant Human FGFR-2 alpha (IIIc)/KGFR/CD332 Protein
The Recombinant Human FGFR-2 alpha (IIIc)/KGFR/CD332 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating FGFR-2 alpha (IIIc)/KGFR/CD332 function and its implications in health and disease.
This product (SKU: RPCB0812) is produced using HEK293 cells and features a C-hFc&His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 66.31 kDa with an observed molecular weight of 100-120 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE;≥ 90 % as determined by HPLC.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Human FGFR-2 alpha (IIIc)/KGFR/CD332 Protein |
| SKU: | RPCB0812 |
| Size: | 10 μg , 20 μg , 50 μg , 100 μg |
| Reactivity: | Human |
| Synonyms: | BBDS, BEK, BFR-1, CD332, CEK3, CFD1, ECT1, JWS, K-SAM, KGFR, TK14, TK25, FGFR2 |
| Tag: | C-hFc&His |
| Expression Host: | HEK293 cells |
| Calculated MW: | 66.31 kDa |
| Observed MW: | 100-120 kDa |
| Gene ID: | 2263 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human FGFR-2 alpha (IIIc)/KGFR/CD332 Protein (RPCB0812), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 0.1 EU/μg of the protein by LAL method. |
| Purity: | ≥ 95 % as determined by SDS-PAGE;≥ 90 % as determined by HPLC. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| Bio-Activity: |
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| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
The protein is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in this gene are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis.
 | Recombinant Human FGFR-2 alpha (IIIc)/KGFR/CD332 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
 | The purity of human FGFR-2 Protein (RPCB0812) was greater than 90% as determined by SEC-HPLC. |
 | Recombinant Human FGFR2 inhibits FGF-acidic dependent proliferation of Balb/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.256-0.991 ng/mL. |
