Description
| Product Name: | Recombinant Human FGFR-2/KGFR/CD332 Kinase Domain Protein |
| SKU: | RPCB0219 |
| Size: | 10µg |
| Tag: | No tag |
| Reactivity: | Human |
| Expression Host: | Baculovirus-Infected Sf9 Cells |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human FGFR-2/KGFR/CD332 Kinase Domain Protein , tested reactivity in Baculovirus-Infected Sf9 Cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 1.0 EU/μg of the protein by LAL method. |
| Purity: | > 97% by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of 20mM Tris, 200mM NaCl, pH 8.0.Contact us for customized product form or formulation. |
| Gene ID: | 2263 |
The protein is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in this gene are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis.
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | P21802 |
 | Recombinant Human FGFR-2/KGFR/CD332 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 35 kDa. |
 | Recombinant Human FGFR-2 inhibits the FGF-acidic dependent proliferation of Balb/c 3T3 mouse fibroblasts. The ED₅₀ for this effect is typically 0.21-0.84 ng/mL, corresponding to a specific activity of 1.19×106-4.76×106units/mg. |
