Granulocyte colony-stimulating factor (G-CSF) is a 20kD glycoprotein with internal disulfide bonds. It promotes the survival, proliferation, and differentiation of neutrophil precursor cells, as well as function activation of mature blood neutrophils. G-CSF is a colony-stimulating factor that is most effective at promoting terminal differentiation of leukemic myeloid cell lines' granulocytes and macrophages. Bacterial endotoxins, TNF, Interleukin-1, and GM-CSF can all induce the synthesis of G-CSF. Prostaglandin E2 inhibits G-CSF production. Interleukin-17 activates secretion of G-CSF in epithelial, endothelial, and fibroblastic cells. Human G-CSF/CSF3 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Recombinant Human G-CSF is produced by our E.coli expression system and the target gene encoding Thr31-Pro204 is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
18.8 kDa
AP Mol Mass:
16 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 10mM HAc-NaAc, 150mM NaCl, 0.004% Tween 80, 5% Mannitol, pH 4.0.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
