Recombinant Human Gamma-glutamyl hydrolase/GGH Protein
The Recombinant Human Gamma-glutamyl hydrolase/GGH Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Gamma-glutamyl hydrolase/GGH function and its implications in health and disease.
This product (SKU: RPCB0111) is produced using advanced expression systems and features a N-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 34.93 kDa with an observed molecular weight of 30-35 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Human Gamma-glutamyl hydrolase/GGH Protein
High quality, high purity and low endotoxin recombinant Recombinant Human Gamma-glutamyl hydrolase/GGH Protein (RPCB0111), tested reactivity in E. coli and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of 20mM HEPES, 150mM NaCl, 1mM TCEP, pH 8.0.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Gamma-glutamyl hydrolase/GGH regulates intracellular folates and antifolates such as methotrexate (MTX) for proper nucleotide biosynthesis and antifolate-induced cytotoxicity, respectively. GGH is a ubiquitously-expressed enzyme that regulates intracellular folate metabolism for cell proliferation, DNA synthesis, and repair. cytoplasmic GGH might play a relevant role in the development and progression of invasive breast cancer.
Recombinant Human Gamma-glutamyl hydrolase/GGH Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
