Cytosolic beta-glucosidase, also known as Cytosolic beta-glucosidase-like protein 1, GBA3, CBG and CBGL1 is a cytoplasm protein which belongs to the glycosyl hydrolase 1 family and Klotho subfamily. GBA3 / CBGL1 is a glycosidase probably involved in the intestinal absorption and metabolism of dietary flavonoid glycosides. GBA3 / CBGL1 is present in small intestine (at protein level). GBA3 / CBGL1 is expressed in liver, small intestine, colon, spleen and kidney. GBA3 / CBGL1 is down-regulated in renal cell carcinomas and hepatocellular carcinomas. GBA3 / CBGL1 is able to hydrolyze a broad variety of glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens. GBA3 / CBGL1 possesses beta-glycosylceramidase activity and may be involved in a nonlysosomal catabolic pathway of glycosylceramide.
Product Name:
Human GBA3/CBGL1 Recombinant Protein (RPES0853)
Product Code:
RPES0853
Size:
10µg
Species:
Human
Expressed Host:
Baculovirus-Insect Cells
Synonyms:
CBG,CBGL1,GBA3,GLUC,KLrP,MGC104276,MGC126878
Accession:
NP_066024.1
Sequence:
Met 1-Leu 469
Fusion tag:
C-His
Activity:
Measured by its ability to hydrolyze 4-methylumbelliferyl-?-D glucopyranoside. The specific activity is >1,500 pmoles/min/µg.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human GBA3 (NP_066024.1) (Met 1-Leu 469) was fused with a polyhistidine tag at the C-terminus.
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
