Recombinant Human Glycophorin-A/GYPA/CD235a protein
The Recombinant Human Glycophorin-A/GYPA/CD235a protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Glycophorin-A/GYPA/CD235a protein function and its implications in health and disease.
This product (SKU: RPCB1765) is produced using HEK293 cells and features a C-hFC tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 33.90 kDa with an observed molecular weight of 45-65 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Human Glycophorin-A/GYPA/CD235a protein
High quality, high purity and low endotoxin recombinant Recombinant Human Glycophorin-A/GYPA/CD235a protein (RPCB1765), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.01 EU/μg of the protein by LAL method
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Bio-Activity:
Anti-GPA Antibody immobilized on CM5 Chip can bind Human GPA, hFc Tag with an affinity constant of 0.72 μM as determined in SPR assay (Biacore T200).
Reconstitution:
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Granulomatosis with polyangiitis (GPA) presents a wide spectrum of manifestations from the common respiratory symptoms to infrequent neurological and cardiac complications. The challenge in diagnosis and management makes the rapidly progressive disorder one of the most challenging dilemmas in clinical medicine.The ultimate goal is an improved prognosis through outcome measures which assesses the disease control with minimal adverse effects of intensive immunosuppressive regimens, an integral part of the clinical approach to improve the quality of life of GPA patients.
Anti-GPA Antibody immobilized on CM5 Chip can bind Human GPA, hFc Tag with an affinity constant of 0.72 μM as determined in SPR assay (Biacore T200).
Recombinant Human Glycophorin-A/GYPA/CD235a protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
