Ganglioside GM2 activator (GM2A) is a small glycolipid transport protein which acts as a substrate specific co-factor for the lysosomal enzyme β-hexosaminidase A (HEXB). HEXB together with GM2A, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. GM2A accommodate several single chain phospholipids and fatty acids, is a lipid transfer protein that stimulates the enzymatic processing of gangliosides, and also T-cell activation through lipid presentation. It extracts single GM2 molecules from membranes and presents them in soluble form to beta-hexosaminidase A for cleavage of N-acetyl-D-galactosamine and conversion to GM3.
Product Name:
Human GM2A Recombinant Protein (RPES1589)
Product Code:
RPES1589
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Ganglioside GM2 activator,Cerebroside sulfate activator protein,GM2-AP,Sphingolipid activator protein 3,SAP-3
Accession:
P17900
Sequence:
Ser32-Ile193
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Ganglioside GM2 activator is produced by our Mammalian expression system and the target gene encoding Ser32-Ile193 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
18.6 kDa
AP Mol Mass:
20 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM TrisHCl,150mM NaCl,pH7.5.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
