Recombinant Human GP100 (HLA-A*02:01) Complex Protein
The Recombinant Human GP100 (HLA-A*02:01) Complex Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating GP100 (HLA-A*02:01) Complex function and its implications in health and disease.
This product (SKU: RPCB1736) is produced using HEK293 cells and features a C-His&Avi tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 50.4 kDa with an observed molecular weight of 52-62 kDa under denaturing conditions, achieving ≥ 95 % as determined by Tris-Bis PAGE;≥ 95 % as determined by HPLC., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Human GP100 (HLA-A*02:01) Complex Protein
SKU:
RPCB1736
Size:
100 μg
Reactivity:
Human
Synonyms:
HLA0201, MHC I, gp100, Glycoprotein 100, ME20-M, P100
Tag:
C-His&Avi
Expression Host:
HEK293 cells
Calculated MW:
50.4 kDa
Observed MW:
52-62 kDa
Protein Description:
High quality, high purity and low endotoxin recombinant Recombinant Human GP100 (HLA-A*02:01) Complex Protein (RPCB1736), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by Tris-Bis PAGE;≥ 95 % as determined by HPLC.
Reconstitution:
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of? the MHC Class I, B2M and YLEPGPVTA peptide of? the gp100.
Recombinant Human GP100 (HLA-A*02:01) Complex Protein was determined by Tris-Bis PAGE under reducing conditions.
The purity of Human GP100 (HLA-A*02:01) was greater than 95% as determined by SEC-HPLC.
