Hepatoma-Derived Growth Factor is a original member of the HDGF family. HDGF is a cytoplasmic protein and contains one PWWP domain. HDGF expression levels are high in the nucleus and cytoplasm of smooth muscle and endothelial cells. HDGF has proliferative, angiogenic and neurotrophic activity. HDGF was initially characterized as a secreted mitogen from the Huh-7 human hepatoma cell line. As a heparin-binding protein, which is highly expressed in tumor cells where it stimulates proliferation. HDGF has mitogenic activity for fibroblasts and acts as a transcriptional repressor. It has been shown that HDGF is linked with tumorigenesis and the growth of cancer.
Product Name:
Human HDGF Recombinant Protein (RPES0664)
Product Code:
RPES0664
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Hepatoma-Derived Growth Factor, HDGF, High Mobility Group Protein 1-Kike 2, HMG-1L2, HDGF, HMG1L2
Accession:
P51858
Sequence:
Met 1-Tyr100
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Hepatoma-Derived Growth Factor is produced by our E.coli expression system and the target gene encoding Met1-Tyr100 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
12.6 kDa
AP Mol Mass:
14 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM Tris, 1mM DTT, 1mM EDTA, pH 7.5.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
