Description
| Product Name: | Recombinant Human HER1/ERBB1/EGFR Protein |
| SKU: | RPCB0751 |
| Size: | 10µg |
| Tag: | C-hFc&His |
| Reactivity: | Human |
| Expression Host: | HEK293 cells |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human HER1/ERBB1/EGFR Protein , tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 0.1 EU/μg of the protein by LAL method. |
| Purity: | > 90% by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| Gene ID: | 1956 |
The EGFR (epidermal growth factor receptor) subfamily of receptor tyrosine kinases comprises four members: EGFR (also known as HER-1, ErbB1, or ErbB), ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4). EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc.The human EGFR cDNA encodes a 1210 amino acid (aa) precursor with a 24 aa signal peptide, a 621 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 542 aa cytoplasmic domain. EGFR signaling has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently, which is overexpressed in a wide variety of tumors and is the target of several anti-cancer drugs.
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | P00533 |
 | Active Recombinant Human EGFR Protein was determined by SDS- PAGE with Coomassie Blue, showing a band at 125-145 kDa. |
 | Immobilized Human EGF at 10 μg/mL (100 μL/well) can bind Human EGFR with a linear range of 0.6-209.9 ng/mL. |
