Description
Recombinant Human HGF receptor/c-MET/MET Protein
The Recombinant Human HGF receptor/c-MET/MET Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating HGF receptor/c-MET/MET function and its implications in health and disease.
This product (SKU: RPCB0852) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 102.47 kDa with an observed molecular weight of 50, 100 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
High Purity by Affinity Chromatography | |
Mammalian & Bacterial Expression Systems | |
High lot-to-lot consistency via strict QC |
Product Name: | Recombinant Human HGF receptor/c-MET/MET Protein |
SKU: | RPCB0852 |
Size: | 10 μg , 20 μg , 50 μg , 100 μg |
Reactivity: | Human |
Synonyms: | HGFR, AUTS9, RCCP2, c-Met, DFNB97, MET, HGFR, hepatocyte growth factor receptor, AUTS9, DFNB97, RCCP2, c-Met |
Tag: | C-His |
Expression Host: | HEK293 cells |
Calculated MW: | 102.47 kDa |
Observed MW: | 50, 100 kDa |
Gene ID: | 4233 |
Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human HGF receptor/c-MET/MET Protein (RPCB0852), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
Endotoxin: | < 0.1 EU/μg of the protein by LAL method. |
Purity: | ≥ 95 % as determined by SDS-PAGE. |
Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
Bio-Activity: | Measured by its binding ability in a functional ELISA. Immobilized recombinant human HGFR at 2 μg/mL (100 μL/well) can bind Rrecombinant human HGF with a linear range of 25-250 ng/mL. |
Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a glycosylated receptor tyrosine kinase (RTK) that has been shown to be overexpressed and/or mutated in a variety of malignancies. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin.