B1 is a member of the HMGB family, which includes three other proteins: HMGB1, HMGB2, and HMGB3. It includes two HMG box DNA-binding domains - box A and B, which are highly negatively charged at their C termini. HMGB1 is a nuclear protein that maintains nucleosomes and permits DNA bending, which is required for gene transcription. Finally, it is shown that HMGB1 can also function as a cytokine extracellularly and regulate monocyte, T cell, dendritic cell activities in inflammatory responses. Human HMGB1 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human HMGB1 Recombinant Protein (RPES2218)
Product Code:
RPES2218
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
High Mobility Group Protein B1, High Mobility Group Protein 1, HMG-1, HMGB1, HMG1
Accession:
P09429
Sequence:
Gly2-Phe89
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human HMGB1 is produced by our E.coli expression system and the target gene encoding Gly2-Phe89 is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
10.5 kDa
AP Mol Mass:
14 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 50mMHepes;500mMNaCl;0.5mMDTT; pH7.9 .
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
