The Recombinant Human IFN-alpha 1/13 (Q114A) Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating IFN-alpha 1/13 (Q114A) function and its implications in health and disease.
This product (SKU: RPCB0145) is produced using E. coli and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 21.01 kDa with an observed molecular weight of 18 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human IFN-alpha 1/13(Q114A) Protein (RPCB0145), tested reactivity in E. coli and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Bio-Activity:
Measured by its ability to stimulate GBP2 expression in 293T human embryonic kidney cells. 0.1-1 ng/mL of Recombinant Human IFNA1 can effectively Stimulating GBP2 expression. 2.Measured by its ability to stimulate human colon adenocarcinoma cells (Caco-2 cells). 10 ng/μL of Recombinant Human IFN-alpha 1/13(Q114A) can upregulate the expression of MDA5.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
IFNA1, also known as IFN-alpha and IFNA, belongs to the alpha/beta interferon family. Interferons(IFNs) are proteins made and released by host cells in response to the presence of pathogens such as viruses, bacteria, parasites or tumor cells.Leukocyte interferon is produced predominantly by B lymphocytes. Immune interferon is produced by mitogen- or antigen-stimulated T lymphocytes. IFNA1 is produced by macrophages and has has both anti-viral and immunomodulatory activities on target cells.
Recombinant Human IFN-alpha 1/13(Q114A) Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Active Recombinant Human IFNA1 stimulates GBP2 expression in 293T human embryonic kidney cells. 0.1-1ng/mL of Recombinant Human IFNA1 can effectively Stimulating GBP2 expression.
