The Recombinant Human IFN-alpha 6 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating IFN-alpha 6 function and its implications in health and disease.
This product (SKU: RPCB0561) is produced using HEK293 cells and features a C-6×His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 20.90 kDa with an observed molecular weight of 15-20 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human IFN-alpha 6 Protein (RPCB0561), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Bio-Activity:
Measured in a cell cytotoxicity assay using TF-1 cells. The ED 50 for this effect is 0.12‑0.48 ng/mL, corresponding to a specific activity of 2.50×10 6 ~8.33×10 6 units/mg.
Reconstitution:
Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Interferon α-6 (IFN-α6) is a secreted protein which belongs to the α/β interferon family. IFN-α6 is produced bymacrophages, expressed at low level, only 1.0% of the average gene in this release. IFN-α6 contains interferonalpha, beta and delta domain. IFN- α has antiviral activities. Interferon stimulates the production of twoenzymes: a protein kinase and an oligoadenylate synthetase.
Recombinant Human IFN-alpha 6 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Recombinant Human IFNA6/IFN-alpha 6/LeIF K Protein cytotoxicity assay using TF-1 cells. The ED50 for this effect is 0.12‑0.48 ng/mL, corresponding to a specific activity of 2.50×106~8.33×106 units/mg.
