The Recombinant Human IgA1 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating IgA1 function and its implications in health and disease.
This product (SKU: RPCB0128) is produced using advanced expression systems and features a N-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 26.27 kDa with an observed molecular weight of 30-40 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Human IgA1 Protein
SKU:
RPCB0128
Size:
100 μg
Reactivity:
Human
Synonyms:
Immunoglobulin heavy constant alpha 1, Ig alpha-1 chain C region, Ig alpha-1 chain C region BUR, Ig alpha-1 chain C region TRO , IGHA1 , IgA1
High quality, high purity and low endotoxin recombinant Recombinant Human IgA1 Protein (RPT0023), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Bio-Activity:
Measured by its binding ability in a functional ELISA. Immobilized Human IGHA1 (Catalog: RPT0023) at 5 μg/mL (100 μL/well) can bind Human CD89/FCAR (RPCB0279) with a linear range of 0.01-7.4 ng/mL.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Recombinant Human IgA1 Protein ，Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen.
Recombinant Human IgA1 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized Human IGHA1 (Catalog: RPT0023) at 5 μg/mL (100 μL/well) can bind Human CD89/FCAR (RPCB0279) with a linear range of 0.01-7.4 ng/mL.
