Immunoglobulin G (IgG) is a monomeric immunoglobulin that is involved in the secondary antibody response and the only isotype that may pass through the human placenta, and it is produced and released by plasma B cells. IgG antibodies protect the body against germs by agglutination and immobilization, complement activation, toxin neutralization, and antibody-dependent cell-mediated cytotoxicity (ADCC). The IgG tetramer comprises two heavy chains (50 kDa) and two light chains (25 kDa), which are linked via disulfide bonds. The two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). Among the four IgG subclasses (IgG1, 2, 3, and 4), IgG1 is most prevalent and binds to Fc receptors (FcR) on phagocytic cells with great affinity. Human IgG1 Fc Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human IgG1 Fc Recombinant Protein (RPES0971)
Product Code:
RPES0971
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
g gamma-1 chain C region,IGHG1
Accession:
P01857
Sequence:
Asp104-Lys330
Fusion tag:
Activity:
Immobilized Human IgG1 Fc at 2µg/ml(100 ?l/well) can bind Human CD64-His(Cat: PKSH033655). The ED50 of Human IgG1 Fc is 47.99 ng/ml .
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Immunoglobulin G1 Fc is produced by our Mammalian expression system and the target gene encoding Asp104-Lys330 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
41.6 kDa
AP Mol Mass:
32 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB,150mM NaCl,pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
