The Recombinant Human IL-1 beta Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating IL-1 beta function and its implications in health and disease.
This product (SKU: RPCB0137) is produced using E. coli and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 18.22 kDa with an observed molecular weight of 18 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human IL-1 beta Protein (RPCB0137), tested reactivity in E. coli and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Bio-Activity:
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED 50 for this effect is 6.04-24.16 pg/mL, corresponding to a specific activity of 4.14×10 7 ~1.66×10 8 units/mg.
Measured by its binding ability in a functional ELISA. Immobilized Human IL-1 beta (RPCB0137) at 10 μg/mL (100 μL/well) can bind Biotinylated Human IL-1R1 (RPCB0850) with a linear range of 0.002-1 μg/mL.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family.This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity.
Recombinant Human IL-1 beta Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Recombinant Human IL-1 beta Protein is greater than 95% as determined by SEC-HPLC.
Recombinant Human IL-1 beta promotes the proliferation of D10.G4.1 mouse helper T cells. The ED50 for this effect is 6.04-24.16 pg/mL, corresponding to a specific activity of 4.14×107~1.66×108 units/mg.
