The Recombinant Human IL-1 Rrp2/IL-1 R6 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating IL-1 Rrp2/IL-1 R6 function and its implications in health and disease.
This product (SKU: RPCB1265) is produced using HEK293 cells and features a C-hFc tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 61.90 kDa with an observed molecular weight of 75-100 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human IL-1 Rrp2/IL-1 R6 Protein (RPCB1265), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Bio-Activity:
Measured by its binding ability in a functional ELISA. Immobilized human IL-36 beta (RPCB0156) at 10 μg/mL (100 μL/well) can bind human IL1RL2(RPCB1265) with a linear range of 0.018-2.62 μg/mL.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
IL1RL2 (Interleukin 1 Receptor-Like 2, also known as IL-36R) is a Protein Coding gene. The protein encoded by this gene is a member of the interleukin 1 receptor family. This gene and four other interleukin 1 receptor family genes form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. The IL36 receptor is an important mediator molecule in the inflammatory response. Animal data suggest that IL1RL2 is involved in mucosal healing. Therapeutic intervention of IL-36R signaling offers an innovative treatment paradigm for targeting epithelial cell-mediated inflammatory diseases such as the life-threatening psoriasis variant called generalized pustular psoriasis (GPP). Diseases associated with IL1RL2 include Familial Cold Autoinflammatory Syndrome 2 and Geographic Tongue.
Recombinant Human IL-1 Rrp2/IL-1 R6 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized human IL-36 beta (RPCB0156) at 10 μg/mL (100 μL/well) can bind human IL1RL2(RPCB1265) with a linear range of 0.018-2.62 μg/mL.
