The Recombinant Human IL-12/IL-12A&IL-12B Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating IL-12/IL-12A&IL-12B function and its implications in health and disease.
This product (SKU: RPCB0906) is produced using advanced expression systems and features a C-His(IL12B)&C-Flag(IL12A) tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 25.54 kDa(IL-12A), 35.54 kDa(IL-12B) with an observed molecular weight of 37 kDa(IL-12A), 40-55 kDa(IL-12B) under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human IL-12/IL-12A&IL-12B Protein (RPCB0906), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Bio-Activity:
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human IL-12 at 1 μg/mL (100 μL/well) can bind Recombinant Human IL12Rβ1 with a linear range of 30.6-122.4 ng/mL.
Measured in a cell proliferation assay using anti-CD3 antibody activated Human T lymphocyte leukemia cells (jurkat). The ED 50 for this effect is typically 0.1-0.4 ng/mL, corresponding to a specific activity of 2.5×10 6 -1.0×10 7 units/mg.
Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED 50 for this effect is 2-8 ng/mL, corresponding to a specific activity of 1.25×10 5 ~5×10 5 units/mg.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Recombinant Human IL-12/IL-12A&IL-12B Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized Recombinant Human IL-12 at 1μg/mL (100 μL/well) can bind Recombinant Human IL12Rβ1 with a linear range of 30.6-122.4 ng/mL.
Recombinant Human IL-12/IL-12A&IL-12B induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 2-8 ng/mL, corresponding to a specific activity of 1.25×105~5×105 units/mg.
