The Recombinant Human IL-17F Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating IL-17F function and its implications in health and disease.
This product (SKU: RPCB0789) is produced using HEK293 cells and features a N-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 15.76 kDa with an observed molecular weight of 18-20 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human IL-17F Protein (RPCB0789), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.01 EU/μg of the protein by LAL method
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.Contact us for customized product form or formulation.
Bio-Activity:
Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells in the presense of 2 ng/mL TNFα(RPCB0855). The ED 50 for this effect is 3.51-14.04 ng/mL, corresponding to a specific activity of 7.12×10 4<
Reconstitution:
Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Recombinant Human IL-17F Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Recombinant Human IL-17F Protein induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells in the presense of 2 ng/mL TNFα(RPCB0855). The ED50 for this effect is 3.51-14.04 ng/mL, corresponding to a specific activity of 7.12×104~2.85×105 units/mg.
