The Recombinant Human IL-23/IL-12B&IL-23A Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating IL-23/IL-12B&IL-23A function and its implications in health and disease.
This product (SKU: RPCB0873) is produced using HEK293 cells and features a No tag(IL12B)&N-his(IL23A) tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 19.50 kDa(IL-12A), 34.71 kDa(IL-12B) with an observed molecular weight of 20 kDa(IL-23A), 45-50 kDa(IL-12B) under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human IL-23/IL-12B&IL-23A Protein (RPCB0873), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Bio-Activity:
Measured by its binding ability in a functional ELISA.Immobilized Human IL-12B&IL-23A at 5μg/mL (100 μL/well) can bind Human IL23R with a linear range of 4-135 ng/ml.
Measured by its ability to induce IL-17 secretion by mouse splenocytes. The ED 50 for this effect is 21.23-84.94 ng/mL, corresponding to a specific activity of 1.17×10 4 ~4.71×10 4 units/mg.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Recombinant Human IL-23/IL-12B&IL-23A Protein was determined by SDS-PAGE under reducing (R) and non-reducing (NR) conditions.
Immobilized Human IL-12B&IL-23A at 5μg/mL (100 μL/well) can bind Human IL23R with a linear range of 4-135 ng/mL.
