The Recombinant Human Insulysin/IDE Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Insulysin/IDE function and its implications in health and disease.
This product (SKU: RPCB1949) is produced using Mammalian expression system and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 114.25 kDa with an observed molecular weight of 120 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Human Insulysin/IDE Protein
SKU:
RPCB1949
Size:
50 μg
Reactivity:
Human
Synonyms:
Insulin-Degrading Enzyme, Abeta-Degrading Protease, Insulin Protease, Insulinase, Insulysin, IDE
High quality, high purity and low endotoxin recombinant Recombinant Human Insulysin/IDE Protein (RP03201LQ), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 15 0mM NaCl, 0.05% Brij35, 10% Glycerol, pH 7.5.
Storage:
Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
Insulin-Degrading Enzyme (IDE) is a secreted enzyme that belongs to the peptidase M16 family. IDE is a large zinc-binding protease and cleaves multiple short polypeptides that vary considerably in sequence. IDE plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin, and other peptides, and thereby plays a role in intercellular peptide signaling. IDE degrades amyloid formed by APP and IAPP. IDE may participate in the degradation and clearance of naturally secreted amyloid β-protein by neurons and microglia. IDE, which migrates at 110 kDa during gel electrophoresis under denaturing conditions, has since been shown to have additional substrates, including the signaling peptides glucagon, TGF α and β-endorphin.
Recombinant Human Insulysin/IDE Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
