Recombinant Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein
The Recombinant Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer function and its implications in health and disease.
This product (SKU: RPCB1530) is produced using HEK293 cells and features a C-His&Avi tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 111.8 kDa(ITGA5), 83.2 kDa(ITGB1) with an observed molecular weight of 100-140 kDa under denaturing conditions, achieving ≥ 95 % as determined by Tris-Bis PAGE;≥ 95 % as determined by HPLC.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein
High quality, high purity and low endotoxin recombinant Recombinant Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein (RPCB1530), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by Tris-Bis PAGE;≥ 95 % as determined by HPLC.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Bio-Activity:
Immobilized Human ITGA5&ITGB1,His Tag at 0.5 μg/mL (100 μL/Well). Dose response curve for Anti-ITGA5&ITGB1 Ab.with the EC 50 of 35.4 ng/mL determined by ELISA.
Reconstitution:
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Recombinant Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein was determined by Tris-Bis PAGE under reducing conditions with Coomassie Blue.
The purity of Human ITGA5&ITGB1 is greater than 95% as determined by SEC-HPLC.
