Interleukin-33 (IL-33) belongs to the IL-1 superfamily. IL33 is highly expressed in endothelial venules found in tonsils; Peyer patches and mesenteric lymph nodes; but almost undetectable in placenta. IL33 induces the production of T helper-2 (Th2)-associated cytokines. IL-33 is a cytokine that mediates its biological effects by binding to and signals through IL1RL1/ST2 and IL-1 Receptor Accessory Protein (IL1RAP); activating intracellular molecules in the NF-?B and MAP kinase signaling pathways that drive production of type 2 cytokines from polarized Th2 cells.
Product Name:
Human Interleukin-33/IL-33 Recombinant Protein (RPES2397)
Product Code:
RPES2397
Size:
20 µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Interleukin-33, IL-33, Interleukin-1 Family Member 11, IL-1F11, Nuclear Factor From High Endothelial Venules, NF-HEV, IL33, C9orf26, IL1F11, NFHEV
Accession:
O95760
Sequence:
Ser112-Thr 270
Fusion tag:
N-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Interleukin-33 is produced by our E.coli expression system and the target gene encoding Ser112-Thr270 is expressed with a 6His tag at the N-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
20.3 kDa
AP Mol Mass:
21 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM Tris;150mM NaCl; 1mM EDTA;pH 8.0.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
