IL-12 is a heterodimeric pleiotropic cytokine composed of a 40 kDa (p40) subunit and a 35 k Da (p35) subunit. The human IL-12 and mouse IL-12 share 70% amino acid identity in their p40 and p35 subunits, respectively. IL-12 is required for the differentiation of naive T cells into Th1 cells. It's a hormone that acts on T cells and can help them grow and function. It inhibits the reduction of interferon gamma (IFN-gamma) production by T cells and natural killer (NK) cells, and it increases IL-4-mediated suppression of IFN-gamma. T cells that produce IL-12 have a coreceptor, CD30, which is associated with IL-12 activity. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 mediates enhancement of the cytotoxic activity of NK cells and CD8+ cytotoxic T lymphocytes. Human Interleukin2/IL2 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human Interleukin2/IL2 Recombinant Protein (RPES1195)
Measured in a cell proliferation assay using antibody against CD3-activated human peripheral blood lymphocytes (PBL). The ED50 for this effect is 0.1-0.5ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Interleukin-12 is produced by our Mammalian expression system and the target gene encoding Arg23-Ser219&Ile23-Ser328 is expressed.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
22.5&34.7 kDa
AP Mol Mass:
40-50 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS, pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
