Description
Recombinant Human Kininogen-1/KNG1 Protein
The Recombinant Human Kininogen-1/KNG1 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Kininogen-1/KNG1 function and its implications in health and disease.
This product (SKU: RPCB1125) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 46.66 kDa with an observed molecular weight of 70 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Human Kininogen-1/KNG1 Protein |
| SKU: | RPCB1125 |
| Size: | 10 μg , 20 μg , 50 μg , 100 μg |
| Reactivity: | Human |
| Synonyms: | BDK, BK, KNG, KNG1, BK, KNG |
| Tag: | C-His |
| Expression Host: | HEK293 cells |
| Calculated MW: | 46.66 kDa |
| Observed MW: | 70 kDa |
| Gene ID: | 3827 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human Kininogen-1/KNG1 Protein (RPCB1125), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 0.1 EU/μg of the protein by LAL method. |
| Purity: | ≥ 95 % as determined by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| Bio-Activity: | Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC. The IC50 value approximately is 1 nM. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
Kininogen-1 (KNG1) is also known as high molecular weight kininogen, Alpha-2-thiol proteinase inhibitor, Fitzgerald factor, Williams-Fitzgerald-Flaujeac factor, which can be cleaved into the following 6 chains:Kininogen-1 heavy chain, T-kinin, Bradykinin, Lysyl-bradykinin, Kininogen-1 light chain, Low molecular weight growth-promoting factor. Kininogen-1 is a secreted protein which contains three cystatin domains. HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII. As with many other coagulation proteins, the protein was initially named after the patients in whom deficiency was first observed.Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis.
 | Recombinant Human Kininogen-1/KNG1 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
 | Recombinant human KNG1 inhibits papain cleavage of a fluorogenic peptide substrate Z-FR-AMC (Catalog#ES009).The IC50 value approximately is 1nM. |
