Lipase family. The gene encoding this protein is expressed mainly in brain, liver and testes,followed by secreting into plasma and cerebral spinal fluid. The esterification of cholesterol is required for cholesterol transport. LCAT is a central enzyme in the extracellular metabolism of plasma lipoproteins. Defects in LCAT are the cause of lecithin-cholesterol acyltransferase deficiency (LCATD) and a cause of fish-eye disease (FED).
Product Name:
Human LCAT Recombinant Protein (RPES3202)
Product Code:
RPES3202
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Phosphatidylcholine-sterol acyltransferase, also named Lecithin-cholesterol acyltransferase, Phospholipid-cholesterol acyltransferase and LACT, is an extracellular cholesterol esterifying enzyme which belongs to the AB hydrolase superfamily.
Accession:
P04180
Sequence:
Phe25-Glu440
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Lecithin-cholesterol acyltransferase is produced by our Mammalian expression system and the target gene encoding Phe25-Glu440 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
48.1 kDa
AP Mol Mass:
66 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 50mM Acetate Buffer pH-4.0.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
