LGMN/Legumain is a cysteine protease with a strict asparaginyl bond hydrolysis specificity. LGMN/Legumain may be involved in bacterial peptides and endogenous proteins' lysosomal/endosomal processing for MHC class II presentation. LGMN seem to be autocatalytic and are activated by an acidic pH. Protein expression occurs in monocytes that differentiate into dendritic cells. After lipopolysaccharide is expressed in mature dendritic cells, a fully developed, functioning enzyme is generated. Overexpression of this gene has been linked to a wide range of solid tumours. There is a pseudogene for this gene on chromosome 13. Only two alternative spliced transcript variants have been verified to be biologically valid, but numerous others have been reported. LGMN is related to two distinct diseases: schistosomiasis and Cst3-related cerebral amyloid angiopathy. Human Legumain/LGMN Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human Legumain/LGMN Recombinant Protein (RPES3243)
Recombinant Human Legumain/Asparaginyl Endopeptidase is produced by our Mammalian expression system and the target gene encoding Ile18-Tyr433 is expressed with a 6His tag at the C-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
48.7 kDa
AP Mol Mass:
56 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 20mM TrisHCl, 150mM NaCl, 10% Glycerol, pH 8.0.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
