Fibronectin type III domain-containing protein 5, the precursor of irisin, is a protein that is encoded by the FNDC5 gene. Human Irisin is synthesized as a 212 amino acid (aa) precursor encoding a type 1 transmembrane protein with a 121 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 39 aa cytoplasmic domain. The ECD of Irisin contains a fibronectin type III domain and multiple glycosylation sites. The ECD is proteolytically cleaved to release the 112 aa soluble Irisin hormone into circulation.Mature human, mouse share 100% sequence identity.Irisin induces expression of peroxisome proliferatoractivated receptor γ coactivator 1α (PGC1α) and uncoupling protein1(UCP1), mitochondrialassociated metabolic proteins. Irisin induces the transition of white adipose tissue into more metabolically active beige adipose tissue.Irisin also regulates neuronal cell differentiation and neurite outgrowth in the brain and is involved in the differentiation of osteoblasts.
Product Name:
Recombinant Human/Mouse/Rat Irisin/FNDC5 (N-6His)
SKU:
RPES6269
Size:
50μg, 10μg
Species:
Human, Mouse, Rat
Synonyms:
FNDC5, Fibronectin type III domain-containing protein 5, Fibronectin type III repeat-containing protein 2, Irisin
Tag:
N-His
Sequence:
Asp32-Glu143
Expression Host:
HEK293 Cells
Accession:
Q8NAU1
Endotoxin:
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Calculated MW:
13.4 kDa
Observed MW:
18-28 kDa
Bio-Activity:
Not validated for activity
Form:
Lyophilized powder
Source:
HEK293 Cells-derived Human/Mouse/Rat Irisin, FNDC5 protein Asp32-Glu143, with an N-terminal His
Formulation:
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Storage:
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 ℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
