Recombinant Human/Mouse/Rat mature Activin A/INHBA Protein
The Recombinant Human/Mouse/Rat mature Activin A/INHBA Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Recombinant Human/Mouse/Rat mature Activin A/INHBA function and its implications in health and disease.
This product (SKU: RPCB1347) is produced using advanced expression systems. The protein exhibits a calculated molecular weight of 12.98 kDa with an observed molecular weight of 15-20 kDa under denaturing conditions, achieving ≥ 90 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Human/Mouse/Rat mature Activin A/INHBA Protein
High quality, high purity and low endotoxin recombinant Recombinant Human/Mouse/Rat mature Activin A/INHBA Protein (RPCB1347), tested reactivity in CHO Cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.01 EU/μg of the protein by LAL method
Purity:
≥ 90 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Bio-Activity:
Measured by its ability to inhibit proliferation of MPC-11 cells. The ED 50 for this effect is 3.215-12.86 ng/mL, corresponding to a specific activity of 7.78×10 4 ~3.11×10 5 units/mg.
Reconstitution:
Centrifμge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
The inhibin beta A subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumor-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta A subunit forms a homodimer, activin A, and also joins with a beta B subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. Finally, it has been shown that the beta A subunit mRNA is identical to the erythroid differentiation factor subunit mRNA and that only one gene for this mRNA exists in the human genome.
Recombinant Human/Mouse/Rat mature Activin A/INHBA Protein was resolved with SDS PAGE under reducing (R) and non-reducing (NR) conditions.
Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is 3.215-12.86 ng/mL, corresponding to a specific activity of 7.78×104~3.11×105 units/mg.
