The Recombinant Human/Mouse/Rat mature BMP-2 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Recombinant Human/Mouse/Rat mature BMP-2 function and its implications in health and disease.
This product (SKU: RPCB1593) is produced using advanced expression systems and features a No tag tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 12.78 kDa with an observed molecular weight of 15-20 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human/Mouse/Rat mature BMP-2 Protein (RPCB1593), tested reactivity in E.coli and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of 0.1% TFA, 30% ACN. Contact us for customized product form or formulation.
Bio-Activity:
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells.The ED 50 for this effect is 24.59-98.36 ng/mL, corresponding to a specific activity of 1.02×10 4 ~4.07×10 4 units/mg.
Reconstitution:
Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
BMP-2 like other bone morphogenetic proteins, plays an important role in the development of bone and cartilage. It is involved in the hedgehog pathway, TGF beta signaling pathway, and in cytokine-cytokine receptor interaction. It is also involved in cardiac cell differentiation and epithelial to mesenchymal transition. Like many other proteins from the BMP family, BMP-2 has been demonstrated to potently induce osteoblast differentiation in a variety of cell types. BMP-2 may be involved in white adipogenesis and may have metabolic effects.
Recombinant Human/Mouse/Rat mature BMP-2 Protein was determined by SDS-PAGE under reducing (R) and non-reducing (NR) conditions.
Recombinant Human/Mouse/Rat mature BMP-2 Protein induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells.The ED50 for this effect is 24.59-98.36 ng/mL, corresponding to a specific activity of 1.02×104~4.07×104 units/mg.
