Recombinant Human/Mouse/Rat mature TGF-beta 3 Protein
The Recombinant Human/Mouse/Rat mature TGF-beta 3 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Recombinant Human/Mouse/Rat mature TGF-beta 3 function and its implications in health and disease.
This product (SKU: RPCB0730) is produced using advanced expression systems and features a No tag tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 12.70 kDa with an observed molecular weight of 13-16 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Human/Mouse/Rat mature TGF-beta 3 Protein
High quality, high purity and low endotoxin recombinant Recombinant Human/Mouse/Rat mature TGF-beta 3 Protein (RPCB0730), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.2 μm filtered solution of 50mM Glycine-HCl, 150mM NaCl, pH 2.5. Contact us for customized product form or formulation.
Bio-Activity:
Measured by its ability to inhibit the IL-4-dependent proliferation of HT-2 mouse T cells. The ED 50 for this effect is 24.2-96.6 pg/mL, corresponding to a specific activity of 1.04×10 7 ~4.17×10 7 units/mg.
Reconstitution:
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Transforming growth factor beta 3(TGFB3) is a member of a TGF - β superfamily which is defined bytheirstructural and functional similarities. TGFB3 is secreted as a complex with LAP. This latent form ofTGFB3becomes active upon cleavage by plasmin, matrix metalloproteases, thrombospondin -1, and a subsetofintegrins. It binds with high affinity to TGF- β RII, a type II serine/threonine kinase receptor. TGFB3 is involvedincell differentiation, embryogenesis and development.It is believed to regulate molecules involved incellularadhesion and extracellular matrix (ECM) formation during the process of palate development. WithoutTGF-β3,mammals develop a deformity known as a cleft palate.
Recombinant Human/Mouse/Rat mature TGF-beta 3 Protein was determined by SDS-PAGE under reducing (R) and non-reducing (NR) conditions.
Measured by its ability to inhibit the IL-4-dependent proliferation of HT-2 mouse T cells. The ED50 for this effect is 24.2-96.6 pg/mL, corresponding to a specific activity of 1.04×107~4.17×107 units/mg.
