Description
| Product Name: | Recombinant Human MPO Protein (Trx Tag) |
| SKU: | RPES8075 |
| Size: | 20μg |
| Tag: | N-Trx |
| Reactivity: | Human |
| Expression Host: | E.coli |
| Accession: | P05164 |
| Sequence: | Cys606-Ser745 |
| Calculated Molecular Weight: | 35.2 kDa |
| Observed Molecular Weight: | 35 kDa |
| Synonyms: | fj80f, wu:fj80f, MPO, 84 kDa myeloperoxidase, 89 kDa myeloperoxidase, EC 1.11.1.7, EC1.11.2.2, fj80f04, mpx, myeloid-specific peroxidase, Myeloperoxidase, Myeloperoxidase heavy chain, Myeloperoxidase light chain, PERM, wu:fj80f04, 84 kDa myeloperoxidase, Myeloperoxidase, 84 kDa myeloperoxidase, 89 kDa myeloperoxidase, EC 1.11.1.7, EC1.11.2.2, fj80f04, MPO, mpx, myeloid-specific peroxidase, Myeloperoxidase, Myeloperoxidase heavy chain, Myeloperoxidase light chain, PERM, wu:fj80f04 |
| Endotoxin: | < 10 EU/mg of the protein as determined by the LAL method |
| Purity: | > 90% as determined by reducing SDS-PAGE. |
Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor , myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils.
| Bio-activity: | Not validated for activity |
| Formulation: | Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol. |
| Reconstitution: | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis. |
| Storage: | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
