The NCF2, also known as NCF-2 and p67phox, is a subunit of the multi-protein NADPH oxidase complex. The latent NADPH oxidase is activated by three enzymes: NCF2, NCF1, and a membrane-bound cytochrome b558. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Chronic granulomatous disease is a condition in which the NCF2 gene, as well as other NADPH oxidase subunit genes, are mutated. Human NCF2/P67phox Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human NCF2/P67phox Recombinant Protein (RPES3557)
Product Code:
RPES3557
Size:
100 µg
Species:
Human
Expressed Host:
Baculovirus-Insect Cells
Synonyms:
NCF-2,NOXA2,P67-PHOX,P67PHOX
Accession:
AAH01606.1
Sequence:
Met 1-Val526
Fusion tag:
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human NCF2 (AAH01606.1) (Met1-Val526) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.
Purity:
> 85 % as determined by reducing SDS-PAGE.
Mol Mass:
59.9 kDa
AP Mol Mass:
60 kDa
Formulation:
Lyophilized from sterile 20mM Tris, 300mM NaCl, pH 8.0
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
