Neurotrophin-3 (NT-3) is a neurotrophic factor that belongs to the NGF family and is structurally similar to beta-NGF, BDNF, and NT-4. The NT3 cDNA sequences a 257-amino acid precursor protein with a signal peptide and proprotein that are removed to generate the 119-amino acid mature NT3. The mature human, murine, and rat NT-3 amino acid sequences are identical. NT-3 selectively promotes the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems. Human Neurotrophin-3/NTF3 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human Neurotrophin-3/NTF3 Recombinant Protein (RPES0375)
Immobilized Human NT-3 at 10ug/ml(100 ?l/well) can bind Human TrkB-His(Cat: PKSH033579). The ED50 of Human NT-3 is 3.777 ug/ml .
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Neurotrophin-3 is produced by our E.coli expression system and the target gene encoding Tyr139-Thr257 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
13.6 kDa
AP Mol Mass:
14 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB, 250mM NaCl, pH 7.2.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
