The Recombinant Human NKG2A&KLRD1/CD159a&CD94 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating NKG2A&KLRD1/CD159a&CD94 function and its implications in health and disease.
This product (SKU: RPCB1539) is produced using HEK293 cells and features a N-His&Avi tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 17.9 kDa (CD94), 18.3 kDa (NkG2A) with an observed molecular weight of 33-48 kDa under denaturing conditions, achieving ≥ 95 % as determined by Tris-Bis PAGE;≥ 95 % as determined by HPLC.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Human NKG2A&KLRD1/CD159a&CD94 Protein
SKU:
RPCB1539
Size:
100 μg
Reactivity:
Human
Synonyms:
CD159 antigen-like family member A, CD159a antigen, CD159a, NKG2A, NKG2-A, NKG2-A/B type II integral membrane protein, NKG2-A/B-activating NK receptor, CD94 antigen, CD94, CD94natural killer cells antigen CD94, Killer cell lectin-like receptor subfamily D member 1, killer cell lectin-like receptor subfamily D, member 1, KLRD1, KP43, NK cell receptor
High quality, high purity and low endotoxin recombinant Recombinant Human NKG2A&KLRD1/CD159a&CD94 Protein (RPCB1539), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by Tris-Bis PAGE;≥ 95 % as determined by HPLC.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Bio-Activity:
Immobilized Human NKG2A&CD94,His Tag at 0.5 μg/mL (100 μL/Well). Dose response curve for Anti-NKG2A&CD94 Ab.with the EC 50 of 8.9 ng/mL determined by ELISA.
Reconstitution:
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Recombinant Human NKG2A&KLRD1/CD159a&CD94 Protein was determined by Tris-Bis PAGE under reducing conditions with Coomassie Blue.
The purity of Human NKG2A&CD94 is greater than 95% as determined by SEC-HPLC.
Serial dilutions of Anti-NKG2A Antibody, hFc Tag were added into Human NKG2A&CD94, His Tag: Biotinylated Human HLA-E*01:03 Complex Tetramer, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 31.8ng/ml.
