The Recombinant Human NSE/ENO2 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating NSE/ENO2 function and its implications in health and disease.
This product (SKU: RPCB1761) is produced using E. coli and features a N-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 55 KDa with an observed molecular weight of - under denaturing conditions, achieving ≥ 80 % as determined by SDS-PAGE., ensuring exceptional quality and consistency for research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human NSE/ENO2 Protein (RPCB1761), tested reactivity in E. coli and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 80 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of 20mM Tris-HCl, 100mM KCl, 5mM MgSO4, pH 7.5.
Reconstitution:
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Gamma-enolase, also known as Enolase 2, belongs to the enolase family. The alpha/alpha homodimer of ENO2 is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. Levels of ENO2 increase dramatically in cardiovascular accidents, cerebral trauma, brain tumors and Creutzfeldt-Jakob disease. ENO2 has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. It binds to cultured neocortical neurons and promotes cell survival in a calcium-dependent manner.
Recombinant Human NSE/ENO2 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
