The Recombinant human Olfactomedin-4/OLFM4 Protein is a high-quality recombinant protein produced for advanced research applications in molecular biology and biotechnology. This protein serves as a critical reagent in various experimental contexts, including functional studies, binding assays, and therapeutic development programs, providing researchers with a standardized and reliable tool for investigating protein function and interactions.
This product (SKU: RPCB1525) is produced using HEK293 cells and features a C-6His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 56.02 kDa with an observed molecular weight of 70-75 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE., ensuring exceptional quality and consistency for research applications.
High quality, high purity and low endotoxin recombinant Recombinant human Olfactomedin-4/OLFM4 Protein (RPCB1525), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Olfactomedin-4 (OLFM4), previously called GC-1 (G-CSF stimulated clone-1) is an secreted glycoprotein that is a member of the Olfactomedin/Noelin family of protein. It is expressed in epithelial cells of the prostate, small intestine, colon, bone marrow, and in several cancers, and is up-regulated in epithelial cells during inflammation. Mature OLFM4 is a 490 amino acid (aa) protein that is secreted mainly in a polymeric form which is held together by disulfide bonds and carbohydrate interactions. Mature human OLFM4 shares 71% aa sequence identity with mature mouse and rat OLFM4. Olfactomedin-4 (OLFM4) interacts with cell surface lectins and cadherins, which helps cell adhesion and cell spreading of NIH3T3 and 293T/17 cells. OLFM4 plays a critical role in regulating progression of prostate cancer and is also novel biomarker for triple-negative breast cancer.
Recombinant human Olfactomedin-4/OLFM4 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
