Ubiquitin thioesterase OTUB1, also known as Deubiquitinating enzyme OTUB1, OTU domain-containing ubiquitin aldehyde-binding protein 1, Otubain-1, Ubiquitin-specific-processing protease OTUB1, OTUB1 and OTB1, is a cytoplasm protein which belongs to the peptidase C65 family. OTUB1 is a hydrolase that can remove conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. OTUB1 is a regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. OTUB1 acts via its interaction with RNF128 / GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 of OTUB1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 of OTUB1 stabilizes RNF128 and promotes anergy. OTUB1 regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). OTUB1 mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. OTUB1 is also capable of removing NEDD8 from NEDD8 conjugates, but with a much lower preference compared to 'Lys-48'-linked ubiquitin.
Product Name:
Human OTUB1/OTB1 Recombinant Protein (RPES1673)
Product Code:
RPES1673
Size:
50µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
HSPC263,OTB1,OTU1
Accession:
Q96FW1-1
Sequence:
Met 1-Lys 271
Fusion tag:
N-His
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the human OTUB1 (Q96FW1-1) (Met 1-Lys 271) was expressed, with a polyhistide tag at the N-terminus.
Purity:
> 97 % as determined by reducing SDS-PAGE.
Mol Mass:
32.8 kDa
AP Mol Mass:
37 kDa
Formulation:
Lyophilized from sterile PBS, 20% glycerol, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
