The Recombinant Human PBEF/Visfatin/NAMPT Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating PBEF/Visfatin/NAMPT function and its implications in health and disease.
This product (SKU: RPCB0743) is produced using E. coli and features a NO-tag tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 55.39 kDa with an observed molecular weight of 60-65 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human PBEF/Visfatin/NAMPT Protein (RPCB0743), tested reactivity in E. coli and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.2 μm filtered solution of 20mM HEPES, 150mM NaCl, pH8.0.Contact us for customized product form or formulation.
Bio-Activity:
Measured in a cell proliferation assay using RPMI 8226 cells. The ED50 for this effect is 1.72‑6.89 ng/mL,corresponding to a specific activity of 1.45×105~5.81×105units/mg.
Reconstitution:
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Pre-B cell colony enhancing factor (PBEF) was originally identified as a cytokine that potentiated the clonalexpansion and differentiation of pre-B cells, but it is also acknowledged to be the ubiquitous intracellularenzyme nicotinamide phosphoribosyltranferase (NAMPT) and the adipokine “ visfatin ”. PBEF is constitutivelyexpressed in the fetal membranes where its greatest expression is in the amnion. It has intracellular andextracellular forms. Most of the intracellular functions of PBEF are due to its role as a Nampt which can induceangiogenesis through upregulation of VEGF and VEGFR and secretion of MCP-1. Extracellular PBEF has beenshown to increase inflammatory cytokines, such as TNF- α, IL-1 β, IL-16, and TGF- β 1. PBEF also increases theproduction of IL-6, TNF- α, and IL-1 β in CD14+ monocyctes, macrophages, and dendritic cells, enhances theeffectiveness of T cells.
Recombinant Human PBEF/Visfatin/NAMPT Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Recombinant Human Visfatin/Nampt stimulates cell proliferation of the RPMI 8226 cells. The ED50 for this effect is 1.72‑6.89 ng/mL,corresponding to a specific activity of 1.45×105~5.81×105units/mg.
