PDGFRA; also known as CD140a; together with the structurally homolog protein PDGFRB (CD140b); are cell surface receptors for members of the platelet-derived growth factor family. They are members of the class III subfamily of receptor tyrosine kinase (RTKs) with the similar structure characteristics of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGFRA is expressed in oligodendrocyte progenitor cells and mesothelial cell; and binds all three ligand isoforms PDGF-AA; PDGF-BB and PDGF-AB with high affinity; whereas PDGFRB dose not bind PDGF-AA. PDGFRA plays an essential role in regulating proliferation; chemotaxis and migration of mesangial cells. Recent studies have indicated that PDGFRA acts as a critical mediator of signaling in testis organogenesis and Leydig cell differentiation; and in addition; particularly important for kidney development. Additionally; PDGFRA is involved in tumor angiogenesis and maintenance of the tumor microenvironment and has been implicated in development and metastasis of Hepatocellular carcinoma (HCC). PDGFRA may represent a potential therapeutic target in thymic tumours. PDGFRA gene amplification rather than gene mutation may be the underlying genetic mechanism driving PDGFRA overexpression in a portion of gliomas.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy
Product Name:
Human PDGFRa/CD140a Recombinant Protein (RPES3778)
Product Code:
RPES3778
Size:
100 µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
CD140A,PDGFR-2,PDGFR2,RHEPDGFRA,Platelet-derived growth factor receptor alpha, PDGFR-alpha, Alpha platelet-derived growth factor receptor,CD140 antigen-like family member A, Platelet-derived growth factor alpha receptor, Platelet-derived growth factor receptor 2
Accession:
NP_006197.1
Sequence:
Met 1-Glu 524
Fusion tag:
C-Fc
Activity:
1. Measured by its ability to bind human PDGF-C in a functional ELISA.2. Measured by its ability to bind mouse PDGF-C in a functional ELISA.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain (Met 1-Glu 524) of human PDGFR? (NP_006197.1) was fused with the Fc region of human IgG1 at the C-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
82.9 kDa
AP Mol Mass:
120-130 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
