Recombinant Human Phospholipase A2/PLA2G1B Protein
The Recombinant Human Phospholipase A2/PLA2G1B Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Phospholipase A2/PLA2G1B function and its implications in health and disease.
This product (SKU: RPCB2041) is produced using advanced expression systems and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 17.20 kDa with an observed molecular weight of 15-20 kDa under denaturing conditions, achieving ≥ 90 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Human Phospholipase A2/PLA2G1B Protein
High quality, high purity and low endotoxin recombinant Recombinant Human Phospholipase A2/PLA2G1B Protein (RPCB2041), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 90 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of 10 mM Tris, 5 mM CaCl2, pH 8.0. Contact us for customized product form or formulation.
Bio-Activity:
Measured by its ability to hydrolyze 1-Hexadecanoyl-2-(1-pyrenedecanoyl)-sn-glycero-3-phosphomethanol. The specific activity is >2500 pmol/min/μg.
Reconstitution:
Please contact us for reconstitution instructions.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Phospholipase A2 (PLA2) plays crucial roles in diverse cellular responses, including phospholipid digestion and metabolism, host defense and signal transduction. PLA2 provides precursors for generation of eicosanoids, such as prostaglandins (PGs) and leukotrienes (LTs), when the cleaved fatty acid is arachidonic acid, platelet-activating factor (PAF) when the sn-1 position of the phosphatidylcholine contains an alkyl ether linkage and some bioactive lysophospholipids, such as lysophosphatidic acid (lysoPA).
Recombinant Human Phospholipase A2/PLA2G1B Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
