Protein Phosphatase 1 Regulatory Subunit 14A (PPP1R14A) belongs to the PP1 inhibitor family. PPP1R14A is mapped to chromosome 19q13.13-q13.2. PPP1R14A binds directly to protein kinase C and casein kinase I. Meantime; PPP1R14A is a phosphorylation-dependent inhibitor of smooth muscle myosin phosphatase. PPP1R14A is the inhibitor of PPP1CA. When phosphorylated; PPP1R14A has over 1000-fold higher inhibitory activity; creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction. In addition; inhibition of PPP1R14A also enhances contraction of smooth muscle in the absence of increment of intracellular Ca2+ concentration.
Product Name:
Human PPP1R14A Recombinant Protein (RPES3265)
Product Code:
RPES3265
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Protein Phosphatase 1 Regulatory Subunit 14A, 17 kDa PKC-Potentiated Inhibitory Protein of PP1, Protein Kinase C-Potentiated Inhibitor Protein of 17 kDa, CPI-17, PPP1R14A, CPI17, PPP1INL
Accession:
Q96A00
Sequence:
Met 1-Pro147
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Protein Phosphatase 1 Regulatory Subunit 14A is produced by our E.coli expression system and the target gene encoding Met1-Pro147 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
17.8 kDa
AP Mol Mass:
22 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 20mM TrisHCl; 0.2mM EDTA; 1mM DTT; 10% Glycerol; pH 8.0.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
