cGMP-Dependent Protein Kinase 1 (PRKG1) belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. PRKG1 contains one AGC-kinase C-terminal domain, two cyclic nucleotide-binding domains, and one protein kinase domain. PRKG1 is mainly expressed in the lung and placenta. PRKG1 acts as a key mediator of the nitric oxide (NO)/cGMP signaling pathway. PRKG1 can phosphorylate many proteins that regulate platelet activation and adhesion, smooth muscle contraction, cardiac function, gene expression, feedback of the NO-signaling pathway, and other processes involved in several aspects of the CNS like axon guidance, hippocampal and cerebellar learning, circadian rhythm, and nociception.
Product Name:
Human PRKG1 Recombinant Protein (RPES0433)
Product Code:
RPES0433
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
cGMP-Dependent Protein Kinase 1, cGK 1, cGK1, cGMP-Dependent Protein Kinase I, cGKI, PRKG1, PRKG1B, PRKGR1A, PRKGR1B
Accession:
Q13976-2
Sequence:
Gly2-Pro686
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human cGMP-Dependent Protein Kinase 1 is produced by our Mammalian expression system and the target gene encoding Gly2-Pro686 is expressed with a 6His tag at the C-terminus.
Purity:
> 80 % as determined by reducing SDS-PAGE.
Mol Mass:
78.8 kDa
AP Mol Mass:
75 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 20mM Tris,150mM NaCl,0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF,20% glycerol,pH8.0.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
