PTP1B, also known as PTPN1, belongs to the protein-tyrosine phosphatase (PTP) family. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTP1B contains 1 tyrosine-protein phosphatase domain and is expressed in many tissues. PTP1B is localized to the cytoplasmic face of the endoplasmic reticulum. PTP1B was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of PTP1B in cell growth control, and cell response to IFN stimulation.
Product Name:
Human PTP1B/PTPN1 Recombinant Protein (RPES1951)
Product Code:
RPES1951
Size:
100 µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
PTP1B,PTPN1
Accession:
NP_002818.1
Sequence:
Glu 2-Asn 321
Fusion tag:
N-His
Activity:
Measured by its ability to dephosphorylate a phosphotyrosine residue in an EGF receptor (aa988-998) phosphopeptide substrate, R&D Systems, Catalog # ES006.The specific activity is > 15 nmoles/min/µg.
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the human PTPN1 (NP_002818.1) (Glu 2-Asn 321) was expressed, with a polyhistide tag at the N-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
38 kDa
AP Mol Mass:
38 kDa
Formulation:
Supplied as sterile 10 mM Hepes, 150 mM NaCl, 1 mM DTT, pH 7.5
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
